Fig. 4

DC260126 down-regulated cytokines in the lung of obese asthmatic mice (A) The protein expression of IL-4 (A), IL-5 (B), and IL-13 (C) in serum was detected by ELISA assay (n = 6 per group). The gene expressions of IL-1β (D), TNF-α (E) and IFN-γ (F) in lung tissue was assessed by real-time PCR (n = 6 per group). The protein secretion of IL-1β (H), TNF-α (I) and IFN-γ (G) in BALFs was detected by ELISA assay (n = 6 per group). The data represent the mean ± S.E.M. from three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the control, ^#P < 0.05, ^##P < 0.01 and ^##P < 0.001 compared with the HDF-OVA group