Fig. 3
From: PEEP application during mechanical ventilation contributes to fibrosis in the diaphragm
Application of PEEP during mechanical ventilation leads to diaphragm fibrosis. A, B Masson’s trichrome staining revealed fibrosis in the diaphragms of the MV + PEEP group. Scale bar = 50 μm. C–E Diaphragms of three groups were detected by qPCR to analyze fibrosis-related gene expression. Values are represented as the mean ± SD, n = 6 (*p < 0.05, **p < 0.01, ***p < 0.001)