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Fig. 6 | Respiratory Research

Fig. 6

From: RAGE inhibition alleviates lipopolysaccharides-induced lung injury via directly suppressing autophagic apoptosis of type II alveolar epithelial cells

Fig. 6

Autophagic cell death happened in A549 cells induced by LPS. The effect of LPS on A549 cells at different concentrations (A) and different time points (B) was detected by CCK-8. The immunoblot of RAGE, Beclin1, LC3II/I and cleaved Caspase 3 in A549 cells, when treated with different concentrations (C) and different time points (D) of LPS. Each experiment was repeated more than three times. E The immunoblot of Beclin1, LC3II/I and cleaved Caspase 3 in A549 cells upon LPS treated when being pretreated with or without 3-MA. 3-MA effectively inhibited the autophagy activation induced by LPS treatment. F The cell survival rate of A549 cells in response to 3-MA pre-treatment followed by LPS stimulation. *Indicates the significant difference compared with the LPS 0 μg/mL group or LPS 0 h group or Control group. # Indicates a significant difference compared with the LPS group. P < 0.05, P-values by Kruskal–Wallis test with Dunn’s post hoc tests. each experiment was repeated more than three times

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Respiratory Research

ISSN: 1465-993X

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