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Fig. 3 | Respiratory Research

Fig. 3

From: O-linked N-acetylglucosamine affects mitochondrial homeostasis by regulating Parkin-dependent mitophagy in hyperoxia-injured alveolar type II cells injury

Fig. 3

OGT increased the expression of Pink1/Parkin, and Parkin might play a negative feedback role on OGT in RLE-6TN cells. a Prediction of potential O-GlcNAcylation sites in rat Parkin protein using YinOYang (http://www.cbs.dtu.dk/services/YinOYang/). b Co-immunoprecipitation analysis of the binding between O-GlcNAc and Parkin. c Western blot analysis of O-GlcNAc, OGA, OGT, and β-actin in Parkin-overexpression and -knockdown RLE-6TN cells. d Gray value analysis of the western blot of O-GlcNAc, OGA, and OGT in Parkin-overexpression and -knockdown RLE-6TN cells. e qPCR analysis of O-GlcNAc, Oga, and Ogt levels in Park2-overexpression and -knockdown RLE-6TN cells. f Western blot analysis of OGT, Pink1, Parkin, LC3BII, and β-actin in Ogt-knockdown RLE-6TN cells. g qPCR analysis of Parkin, Oga, and Bax expression after Ogt knockdown in RLE-6TN cells. Data are shown as the mean ± SEM (n = 6) and were analyzed using two-way ANOVA followed by Tukey post-hoc tests. *P < 0.05, **P < 0.01. NC: Negative control; OV: overexpression; Sh RNA: short hairpin RNA; Si RNA: short interfering RNA

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Respiratory Research

ISSN: 1465-993X

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