Fig. 6

DSF inhibits pyroptosis of HPASMCs under hypoxic conditions. A PI staining of pyroptotic HPASMCs under hypoxic conditions was significantly reduced after DSF treatment (10 μmol/L). Red: propidium iodide (PI); Blue: Hoechst 33,342. Scale bars = 100 µm (n = 5). B DSF reduced the release of LDH in HPASMCs subjected to hypoxia for 24 h (n = 4). C Representative WB images and analysis of caspase-1 and CI. caspase-1 (n = 3). D Representative WB images and analysis of GSDMD and N-GSDMD (n = 3). E Representative WB images and analysis of NLRP3 and ASC (n = 3). F, G Representative WB images and analysis of IL-18 and IL-1β (n = 3). H, I ELISA detection of the inflammatory factors IL-1β and IL-18. Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and ^#P < 0.05 vs. normoxia