Fig. 4

DSF inhibits the formation of N-GSDMD and the release of IL-18 and IL-1β in the lung tissue of PH mice. A Representative WB images and analysis of caspase-1 and CI. caspase-1 and IL-1β (n = 5 each). B Representative WB images and analysis of GSDMD, N-GSDMD and IL-18 (n = 5 each). C Representative WB images and analysis of NLRP3 and ASC. D, E ELISA detection of inflammatory factors (IL-18 and IL-1β) in the plasma, (n = 6 each). Values are the mean ± SEM. Statistical significance was analysed by one-way ANOVA followed by Bonferroni’s multiple comparison test or the Kruskal–Wallis test. *P < 0.05 vs. vehicle, and ^#P < 0.05 vs. normoxia