Fig. 6

Relatively increased neutrophil recruitment to the lungs during lung injury coincides with worse weight loss in mice in Facility B. Lungs were harvested from progenies of the conventionalized ex-GF founders in the two facilities after bleomycin (1 U/kg) or PBS (vehicle control) challenge. A Normalized frequencies of neutrophils in the lungs on day 3 post-BLM. Data from three independent experiments were combined, and experimental values were normalized by the group variance for each experiment. The cells were gated based on Ly6G+CD11b+lineage− as shown in Additional file 1: Fig. S5C. P-values were obtained from Sidak’s multiple comparisons test after confirming P < 0.05 from one-way ANOVA. B Fold change of neutrophil accumulations in lungs on 3 days after bleomycin challenge compared to PBS treatment in each facility. P-values are depicted as P < 0.05 (*), P < 0.01 (**), P < 0.001 (***)