Fig. 5
From: Gut microbiota modulates bleomycin-induced acute lung injury response in mice
Differential colonization of commensal species corresponds with specific immune landscapes of the lungs in naĂ¯ve animals. Gut-draining LN and lungs of unperturbed progenies of the conventionalized ex-GF founders were immunophenotyped. A Frequencies of RORγt+ Treg (CD25+Foxp3+) cells in gLN. B Frequencies of Th17 (RORγt+Foxp3−) cells in gLN. C, D Non-adaptive immune cell population (CD45+CD3−CD19−) in the lungs were analyzed using the FlowSOM clustering algorithm. Total 128,000 cells were analyzed from n = 8 mice from each facility (Down sampled to 8000 cells per mouse). FlowSOM generated 10 clusters with unique phenotypes. C Proportion of cells in each cluster is compared between mice in the two facilities. Multiple unpaired t-tests using the two-stage step-up method were performed for statistical analysis. D Heatmap of expressions of 9 markers in each cluster. E Frequencies of NK cells in the lungs based on manual gating (NK1.1+lineage−). F Frequencies of neutrophils in the lungs (Ly6G+CD11b+lineage−). P-values were obtained from student t-tests and depicted as P < 0.05 (*), P < 0.01 (**), P < 0.001 (***)