Fig. 3
From: Gut microbiota modulates bleomycin-induced acute lung injury response in mice
Gut microbial community structures are different between the two SPF facilities. A Following the 14-day conventionalization, fecal samples were collected from ex-GF mice at before (D0) and after (D7) bleomycin challenge (n = 7 per condition). Gut microbial structures were analyzed by 16S rRNA gene (V4 region) sequencing of the fecal samples. B Weighted UniFrac analysis of similarity coefficients were calculated from 16S rRNA gene sequences of each mouse. Permutational multivariate analysis of variance was done to analyze paired statistical differences in beta diversity. C The within-sample richness and evenness (alpha diversity) were measured by Shannon diversity index [A0 vs. B0 (p-value = 0.0003); A7 vs. B0 (p-value = 0.0267)]. D Differential abundance of microbial taxa between the two facilities at D0 was analyzed using the Statistical Analysis of Metagenomic Profiles (STAMP) package. P-value of 0.05 was used as a cutoff