Fig. 5

CB2R regulates NLRP3 inflammasome activation in PA pneumonia. C57BL/6 J WT mice received either vehicle (V) or JWH133 (J) and were exposed to PBS or PA. Lung NLRP3 inflammasome activation was assessed by measuring the protein levels of NLRP3, ASC, and (p20) caspase-1. Representative immunoblot images are shown in panel (a) and densitometry data are presented in panel (b). CB2KO and WT mice received JWH133 and were exposed to PBS or PA and the protein levels of NLRP3, ASC, and (p20) caspase-1 were analyzed by immunoblot. Representative immunoblot images are shown in panel (c) and densitometry data are presented in panel (d). n = 5, **p < 0.01***p < 0.001, ****p < 0.0001. Data are presented as mean ± SEM