Fig. 4

CB2R regulates PA-induced neutrophil activation. a Representative Immunostaining image of MPO (red) in WT mice lung pre-treated with vehicle (WT-V) or JWH133 (WT-J) and CB2KO mice lung pre-treated with JWH133 (CB2KO-J) and exposed to PBS (control) or PA for 24 h. T1α staining (green) was used to indicate the epithelial cells. BALF was collected at 24 h after PA infection and the release of CitH3 and H2B were analyzed by immunoblot. Representative immunoblot (b) and quantification (c) of CitH3 and H2B in BALF from WT mice pretreated with vehicle or JWH133 and exposed to PBS (control) or PA. Representative immunoblot (d) and quantification (e) of CitH3 and H2B in BALF from WT Vs CB2KO mice pretreated with vehicle or JWH133 and exposed to PBS (control) or PA. n = 4, **p < 0.01***p < 0.001, ****p < 0.0001. Data are presented as mean ± SEM