Fig. 2

JWH133 treatment reduces PA-induced lung inflammation in mice. C57BL/6 J WT mice were treated with vehicle or JWH133 or SR144528 + JWH133 and exposed to either PBS (control) or PA. BALF was collected after 24 h and total cell number (a), and neutrophil population (b) were determined. The levels of inflammatory cytokines IL-1β (c), IL-6 (d), TNF-α (e), and chemokines KC (f) and MIP-2(g) were tested. Mouse primary AMs were exposed to PA and the levels of IL-1β (h) and TNF-α (i) in the cell culture supernatant were evaluated after 16 h of PA exposure. n = 5–8, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are presented as mean ± SEM