Fig. 1

Pharmacological activation of CB2R ameliorates PA-induced alveolar damage and decline in lung function. C57BL/6 J WT mice were treated with vehicle (V) or JWH133 (J) or SR144528 (SR) + JWH133 (J) and exposed to either PBS (control) or PA. Lung injury was assessed after 24 h. Total cell number (a) and total protein content (b) in the BALF were determined from the mice treated with varying doses of JWH133 and exposed to PA infection (0 mg/kg JWH133:vehicle). c Representative H&E images of mice lungs. Total protein content in the BALF (d) was determined. e The lung bacterial burden was evaluated by measuring the bacterial load in the whole lung. f Tissue dampening (G), g tissue elastance (H), and, h static lung compliance (Cst) were examined using the FlexiVent system. n = 5–8, **p < 0.01, ***p < 0.001, ****p < 0.0001. Data are presented as mean ± SEM