Fig. 6From: Preclinical development of a long-acting trivalent bispecific nanobody targeting IL-5 for the treatment of eosinophilic asthmaLarge-scale production of the trivalent IL-5-HSA Nb and its preliminary developability analysis. A The protein expression of the trivalent IL-5-HSA Nb was detected through SDS-PAGE, followed by affinity chromatography, hydrophobic chromatography and molecular sieve chromatography. The cropping gel of purified trivalent IL-5-HSA Nb is displayed. B The protein titer and wet cell weight in the fermentation tank were both measured at the indicated times. C The purity of the trivalent IL-5-HSA Nb was determined through SEC-HPLC analysis. D The stability of the trivalent IL-5-HSA Nb under different temperatures and 3 freeze‒thaw cycles was detected by SEC-HPLC and CEX-HPLCBack to article page