Fig. 2
From: ColdZyme® protects airway epithelia from infection with BA.4/5
Disruption of epithelial integrity by BA.1 and BA.4/5 can be avoided by ColdZyme® pre-treatment. a Pseudostratified epithelia were infected by apical addition of SARS-CoV-2 BA.1 and BA.4/5 (MOI 0.01) with or without ColdZyme® pretreatment and incubated for 72 h. TEER was measured on 1 dpi (left) and 3 dpi (right) using a EVOM volt-ohm-meter. TEER in Ω/cm2 was determined for all conditions (UI, BA.1, BA.1 + ColdZyme, BA.4/5, BA.4/5 + ColdZyme) and plotted on a bar graph. Bars represent the mean + SD from 3 independent pseudostratified epithelia measured in triplicates. Statistical significance was calculated using One-way ANOVA with Tukey´s multiple comparisons test. b Viral RNA was measured from UI, BA.1, BA.1 + ColdZyme, BA.4/5, and BA.4/5 + ColdZyme cultures of pseudostratified epithelia on 3 dpi. The experiment was repeated at least 3 times and statistically significantl differences were determined by one-way ANOVA with Tukey´s multiple comparisons test. All values are means ± SD