Fig. 7
From: PKD1 deficiency induces Bronchiectasis in a porcine ADPKD model
Components of extracellular matrix inPKD1+/−pig lungs were altered. (A) Real-time PCR of genes related to extracellular matrix in 2-year-old pig lungs. (B) Representative Western blot analyses of COL1A2 and COL4A2 and their quantifications. (C) Masson staining of 2 years old pig airways. Scale bar: 100 μm. (D) Real-time PCR of MMP1 and MMP9. n = 3. (E&F) Real-time PCR analyses of genes related to extracellular matrix and α-SM expression in control and Collagen I treated MRC5 cells. (G) Representative Western blot analyses of RHOA/ROCK and ERK signaling expression in control and Collagen I treated MRC5 cells. The bars represent the mean ± SD; *P < 0.05; **P < 0.01; ***P < 0.001.