Fig. 5

Depleting FoxP1 by RNA interference reduced the viability of lung epithelial cells exposed to cigarette smoke extract. Lung epithelial cells were transfected with dsi RNA targeting FoxP1 mRNA (dsi FoxP1) or non-sense mRNA (dsi Scramble). 24h later cells were exposed to 5% cigarette smoke extract overnight and then stained with Zombie Aqua blue (stains non-viable cells). Flow cytometry was performed (gating strategy per A) on 3 biological replicates to record 10,000 events per sample. Cells transfected with dsi FoxP1 showed lower viability than cells transfected with dsi Scramble (B). The differences in % of dead cells were statistically significant (C). Non-parametric tests were used (Mann–Whitney U). *p<0.05