Fig. 1

High throughput sequencing PBMCs of asthmatic children and 10 miRNAs related to Th cell differentiation. Peripheral blood was collected from asthmatic children (n = 5) and non-asthmatic children (n = 5), and PBMCs were isolated. High throughput sequencing was used to screen different expression of miRNAs. There were 506 miRNAs with fold change > 1.5 and P < 0.05, among which 268 up regulated and 238 down regulated (A). GO analysis showed that these aberrant dysregulated miRNAs were related to DNA binding, transcription factor activity, cell inflammation, cell activation, proliferation and so on (B). KEGG signal pathway analysis showed that these miRNAs were closely related to Th cell differentiation, and their expression was down regulated in asthmatic children (C). Through informatics analysis and published database, 10 miRNAs (5 up-regulated and 5 down-regulated) involved in Th cell differentiation were selected (D–F). Among them, miR-493-5p was selected due to large differences between groups and small differences within groups (G)