Fig. 4

Cells from draining lymph nodes of mice with CD19‑driven S1P₁ deletion generate MSS-specific IgGs. Mice were exposed to saline or MSS as described in methods and lymph nodes were harvested 24 h after the last instillation. Cytometry was done as described in Fig. 2B to measure (A) Numbers of mLN B cells and (B) the frequency of CD69⁺ B cells. C) MSS-specific IgGs were assessed by indirect ELISA on mLN B cells supernatant diluted 1:2 and on different (D) BALF and (E) plasma dilutions. mLN: mediastinal lymph node. O.D.: optical density. MSS: Methanosphaera stadtmanae. Averages ± SEM. n = 3–6. *p < 0.05