Fig. 5

FGF10 increases the intensity of glycocalyx components in murine COPD model. Representative immunofluorescence staining images of heparan sulfate (A), chondroitin sulfate (C) and syndecan-1 (E) in mice lungs. Heparan sulfate, chondroitin sulfate and syndecan-1 antibodies were used as glycocalyx specific antigens, and CD31 was used as the mark of endothelial cell. Nuclei were visualized with 4ʹ,6-diamidino-2-phenylindole (DAPI). Scale bar = 50 μm. B, D, F Quantitative analysis of fluorescence intensity was performed (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001. n.s., not significant