Fig. 4
FGF10 attenuates the development of emphysema induced by cigarette smoke extract in mice. A Male C57BL/6 mice (6–8 weeks) were treated with 400 μl cigarette smoke extract (CSE) intraperitoneally once a week for 4 weeks, with different doses of FGF10 administration (10 μg/kg, 100 μg/kg, 1 mg/kg, 2.5 mg/kg) intravenously 24 h before CSE injection. All mice were sacrificed at Day 28. Representative haematoxylin and eosin (H&E) staining images of airspace. Scale bar = 50 μm. Alveolar size was evaluated by mean linear intercept (MLI) (n = 10). B Mice were administered standard saline solution or 240 μg of KLAKLAK2 intraperitoneally once per week for 4 weeks, with different doses of FGF10 administration intravenously 24 h prior to CSE injection. Representative H&E staining images of airspace. Scale bar = 50 μm. Alveolar size was evaluated by mean linear intercept (MLI), (n = 8–10). C Serum glycocalyx component levels in mice from each group detected by ELISA (n = 6–8). *P < 0.05, **P < 0.01, ***P < 0.001. n.s., not significant