Fig. 6

A Representative images of NIH3T3 transfected with or without siRNA. B The number of NIH3T3 in RGS2-siRNA-transfected and control groups (n = 3). C Top: Representative Western blot of RGS2 protein expression in Rgs2-siRNA-transfected groups and control groups of NIH3T3. Bottom: The results are expressed as a bar graph that illustrates RGS2 protein level of Western blot, measured as the RGS2/β-actin protein ratio. The results are presented as means ± SEM (n = 3). D The level of RGS2 mRNA was evaluated by RT-PCR presented as means ± SEM (n = 3). F, G, H The mRNA levels of main pulmonary fibrosis markers (Col1a1, Col1a2, and α-SMA) in Rgs2-siRNA-transfected groups and control groups of NIH3T3 without or with PFD or/and hUC-MSCs treatment. Values are means ± SEM (n = 3). *p < 0.05; **p < 0.01; ***p < 0.01