Fig. 4

The RGS2 mRNA and protein expression levels in mouse lung tissue and cells of different groups. A The RGS2 mRNA levels detected by RT-PCR in mouse lung tissue of different groups (n = 3). B The RGS2 protein expression levels detected by Western blot in mouse lung tissue of different groups (n = 3). C The results are expressed as a bar graph that illustrates RGS2 protein expression, measured as the RGS2/β-actin protein ratio of these groups. The results are presented as means ± SEM (n = 3). D, E HLF-9 (D) and NIH3T3 (E) were treated with various concentrations of PFD (0–10 mM) for 2 h and were then harvested for RT-PCR analysis of RGS2 and β-actin × 1000 mRNA levels. F, G HLF-9 (F) and NIH3T3 (G) were treated with 8 mM PFD over 24 h and were then harvested for RT-PCR analysis of fibrosis marker mRNA levels (n = 3). H Schematic diagram of single culture and co-culture of hUC-MSCs and MFBs. *p < 0.05; **p < 0.01; ***p < 0.01