Fig. 3

Macrophage Lkb1 does not play a role in the host defense during pneumonia caused by encapsulated S. pneumoniae. Mice were inoculated intranasally with approximately 5 × 10⁴ colony-forming units (CFUs) of encapsulated Spneu 6303 and euthanized 12 and 40 h thereafter for determination of bacterial loads (CFUs per milliliter) in the lung and blood (A), the extent of lung inflammation scored on haematoxylin and eosin stained tissue sections as total pathology score (B), and cytokine and chemokine levels (TNFα, IL-1β, IL-6, IL-10, CXCL1, CXCL2 and MPO) in the lung (C). Data are shown as bar graphs with mean ± SD representing 6–8 mice per group at each time point. Groups were compared using the Mann–Whitney U test. All comparisons were not significant