Fig. 2

Macrophage Lkb1-deficient mice show reduced neutrophil influx and cytokine release into the airways on LTA challenge. Cell counts and phenotypes in lungs (A), BALF (B, C) and cytokine concentrations in BALF 6 h after intranasal inoculation with 100 μg LTA. Numbers of neutrophils and alveolar macrophages (AMs) in the lung (A) and BALF (B) after LTA challenge. Neutrophils were gated on CD11c^negLy6G^pos cells. AM subtypes: “classic” AMs (cAMs) (CD11c^posSiglecF^highCD11b^neg), “non-classic” AMs (ncAMs) (CD11c^posSiglecF^lowCD11b^pos) and interstitial macrophages (IMs) (SiglecF^neg and CD11b^high). C Total number and fractional contribution of cAMs and ncAMs in BALF. D Cytokine and chemokine concentrations in BALF. Data are shown as bar graphs showing mean ± SD, representing 8 mice per group. Cell counts and cytokine levels of the Stk11-ΔM mice were compared to littermate controls using multiple student t-test for cells counts (A–C) and the Mann–Whitney U test for cytokine levels (D). *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001