Fig. 6

Intranasal administration of si-IL-24 or IL-37 reduced HDM-induced EMT markers in an HDM-sensitized asthma murine model. Representative immunoblot analysis (a) and quantification (b) of E-cadherin, vimentin and α-SMA protein expression in the lungs of mice. c–f Representative immunofluorescence images of α-SMA, vimentin and IL-24 expression in the lungs of mice. Scale bar = 100 μm (× 400). g and h Representative immunohistochemistry images of E-cadherin expression in the lungs of mice (n = 5 random fields per group). Scale bar = 100 μm (× 630). Bar diagrams and data are presented as the mean ± standard deviation (SD). * vs. PBS group; & vs. HDM group. *^,&P < 0.05; **^,&&P < 0.01; ***^,&&&P < 0.001, ****^,&&&&P < 0.0001. si-NC siRNA targeting of negative control