Fig. 6

Comparison of cigarette smoke-related human in-vivo studies with the current in-vitro study. Expression of the genes that were differentially expressed in the A, B acute smoke exposure study is shown. Only two genes were upregulated and one gene was downregulated in the study. Gene set variation analysis (GSVA) of DEGs identified in in-vivo studies of C, D chronic smokers, and E, F smoking cessation is depicted here. The vertical bar indicates the standard deviation (n = 8). Gene sets which were significantly different after cigarette smoke exposure compared to Air (p < 0.05) are shown by the * symbol. *p < 0.05, ***p < 0.001. G UMAP of the identified cell-types of single-cell RNA-sequencing study of current smokers and never smokers, and H average expression of the DEGs identified in the in-vivo study