Fig. 4

The ratios of immunoreactive ACE protein (using multiple mAbs) to ACE activity. Plasma samples from 20 pulmonary patients were analyzed for ACE activity using ZPHL as a substrate (A). The amount of immunoreactive protein ACE protein with mAb 9B9 (B), 3A5 (C) and i1A8 (D) was divided by mean ACE activity values for both substrates. Bar color scheme is as described in Fig. 1. Data are expressed as % of ACE levels (with different mAbs)/ACE activity ratio from value for control pooled sera samples from healthy controls. Mean values from 2 experiments (each made in triplicates); SD (not shown for clarity) was less than 10%. Patients that have decreased ratios for each mAb (less than 80% of control plasma) were marked with yellow. Patients having decreased ratio for all three mAbs were marked with blue. Patients that were taking ACE inhibitors (revealed as in Fig. 2) were marked with red