Fig. 6
From: ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10
ZC3H4 is involved in regulating the silica-induced release of IL-10 by monocytes. A Representative Western blot showing that SiO2 induced ZC3H4 expression in a time-dependent manner in THP-1 cells. B Densitometric analyses of ZC3H4 protein expression levels in five independent experiments; *P < 0.05 vs. the 0 h group. C Representative immunocytochemical staining images showing that SiO2 induced ZC3H4 expression in THP-1 cells. Scale bar, 100 μm. D Representative Western blot showing that ZC3H4 protein was knocked down in THP-1 cells after plasmid transfection. E Densitometric analyses of ZC3H4 protein expression levels in five independent experiments; *P < 0.05 vs. the corresponding group in the control group. #P < 0.05 vs. the Con-NIC+ ZC3H4-NIC− group and the SiO2 group. F ELISA analysis showing that knocking down the ZC3H4 protein increased IL-10 protein release by THP-1 cells. *P < 0.05 vs. the corresponding group and the control group. #P < 0.05 vs. the Con-NIC+ ZC3H4-NIC− group and the SiO2 group