Fig. 2
From: ZC3H4 regulates infiltrating monocytes, attenuating pulmonary fibrosis through IL-10
Monocytes play a negative role in fibroblast activation, migration and viability after silica treatment. A Representative Western blot showing the effect of CM on the upregulation of COL1A1 and ACTA2 in fibroblasts. B Densitometric analyses of COL1A1 levels in three independent experiments; *P < 0.05 vs. the control group. C Densitometric analyses of ACTA2 levels in three independent experiments; *P < 0.05 vs. the control group. D Representative images of the gel contraction assay of fibroblasts treated with CM. E Gel contraction assay results demonstrating the conditioned medium-induced decrease in fibroblasts; *P < 0.05 vs. the control group. F CCK-8 assay results showing that CM attenuated fibroblast viability; *P < 0.05 vs. the corresponding time point in the control group, n = 3. G Representative images of a scratch assay showing that the migration of fibroblasts was attenuated by CM. Scale bar, 20 μm. H Quantification of the scratch gap distance in three independent experiments; *P < 0.05 vs. the corresponding time point in the control group