Table 2 Primary antibodies and immunohistochemical conditions used for identification of inflammatory cells and biomarkers of humoral immunity in bronchial biopsies
Antibody | Code | Species | Concentration |
|---|---|---|---|
Neutrophilic elastase (neutrophils) | Dako, M752 | Mouse | 1:100 |
CD68 (macrophages) | Dako, M814 | Mouse | 1:300 |
ECP (eosinophils) | Pharmacia, EG2 | Mouse | 1:100 |
Mast cells (tryptase) | Invitrogen, MA538007 | Rabbit | 1:400 |
CD4 | Santa Cruz, SC-19641 | Mouse | 1:40 |
CD8 | Thermo Fischer, RM 9116 | Rabbit | 1:80 |
CD20 | Dako, M755 | Mouse | 1:200 |
NK (natural killer) | Dako, M1014 | Mouse | 1:50 |
Plasma cells | Dako, M7077 | Mouse | 1:25 |
IgA | Gene Thex, GTX22411 | Rabbit | 1:200 |
IgA secretory | Abcam, AB17921 | Mouse | 1:50 |
pIgR | Invitrogen, PAS35340 | Rabbit | 1:20 |