Fig. 6

HSP110 activates YAP/TAZ-TEAD4 pathway in HPASMCs. a Immunofluorescence staining of YAP (red) in HPASMCs received with shHSP110 or shNC after 24 h hypoxic exposure in each group. White scale bars, 50 μm; Yellow scale bars, 25 μm. b–e Protein level p-YAP, YAP, p-TAZ, TAZ and nuclear TEAD4 and quantitative analysis of relative protein ratio of p-YAP/t-YAP and p-TAZ/t-TAZ and relative protein level of TEAD4. Data are means ± SD from 3 biological replicates. In c–e, *p < 0.05 compared to the normoxia group. ^#p < 0.05 compared to the hypoxia-shNC group. f-i HPASMCs received with adenovirus vector carring HSP110 cDNA or control vector after 24 h hypoxic exposure. Protein levels p-YAP, t-YAP, p-TAZ, t-TAZ and nuclear TEAD4 and quantitative analysis of relative protein ratio of p-YAP/t-YAP, p-TAZ/t-TAZ and relative protein level of nuclear TEAD4. Data are means ± SD from 3 biological replicates. In g–i, *p < 0.05 compared to the normoxia group. ^#p < 0.05 compared to the hypoxia-Vector group