Fig. 4

HSP110 affects hypoxia-induced proliferation and migration in HPASMCs. a The identification of HPASMCs by α-SMA staining (N = 1). Scale bars, 50 μm. Relative mRNA level (b) and protein level (c, d) of HSP110 in HPASMCs received with shHSP110 or shNC after 24 h hypoxic exposure (N = 3). e Cell proliferation of HPASMCs in each group after different time hypoxic exposure (N = 3). Wound healing assay (f) and quantification of migration ratio (g) of HPASMCs in each group (N = 3). Scale bars, 200 μm. Data are means ± SD from 3 biological replicates. In b, d, e and g, *p < 0.05 compared to the normoxia group. ^#p < 0.05 compared to the hypoxia-shNC group. Relative mRNA level (h) and protein level (i, j) of HSP110 in HPASMCs received with adenovirus vector carring HSP110 cDNA or control vector after 24 h hypoxic exposure (N = 3). k Cell proliferation of HPASMCs in each group after different time hypoxic exposure (N = 3). Wound healing assay (l) and quantification of migration ratio (m) of HPASMCs in each group (N = 3). Scale bars, 200 μm. Data are means ± SD from 3 biological replicates. In h, j, k and m, *p < 0.05 compared to the normoxia group. ^#p < 0.05 compared to the hypoxia-Vecotor group