| | C3M | PRO-C1 | PRO-C3 | PRO-C6 | FBN-C |
|---|
Mean (ng/mL) | ± SEM | n | Mean (ng/mL) | ± SEM | n | Mean (ng/mL) | ± SEM | n | Mean (ng/mL) | ± SEM | n | Mean (ng/mL) | ± SEM | n |
|---|
Controla | 0.75 | 0.05 | 41 | 27.11 | 4.39 | 29 | 15.07 | 1.16 | 43 | 1.22 | 0.19 | 27 | 47.96 | 9.34 | 38 |
Nintedanib 0.01 µM | 0.65 | 0.04 | 39 | 22.94 | 4.16 | 27 | 14.75 | 1.49 | 41 | 1.13 | 0.22 | 25 | 45.90 | 11.18 | 35 |
Nintedanib 0.03 µM | 0.70 | 0.05 | 39 | 20.33 | 3.93 | 27 | 13.79 | 1.09 | 41 | 1.05 | 0.19 | 25 | 41.85 | 10.65 | 37 |
Nintedanib 0.1 µM | 0.63 | 0.05 | 41 | 25.29 | 3.93 | 29 | 14.42 | 1.11 | 43 | 1.07 | 0.17 | 27 | 45.46 | 9.86 | 38 |
Nintedanib 0.3 µM | 0.57* | 0.04 | 41 | 22.46 | 3.99 | 29 | 13.77 | 1.17 | 43 | 1.19 | 0.19 | 27 | 44.52 | 9.72 | 38 |
Pirfenidone 100 µM | 0.72 | 0.06 | 39 | 23.61 | 3.95 | 27 | 14.28 | 1.02 | 41 | 1.25 | 0.20 | 25 | 45.61 | 9.46 | 36 |
- aDMEM/F12 media containing antibiotics. Data are shown as mean extracellular matrix turnover marker concentration of all available supernatants of all incubated slices from all donors per group ± SD. C3M, PRO-C3 and FBN-C concentration was explored in (n) samples of 10 donors, PRO-C1 and PRO-C6 in (n) samples of 7 donors. Sufficient supernatant was not available to test all markers in some cases, so numbers vary. *P < 0.05 versus control. C3M, neo-epitope of MMP-9 mediated degradation of type III collagen; DMEM, Dulbecco’s modified Eagle’s medium/nutrient mixture; F-12, F-12 Ham; FBN-C, C-terminal of fibronectin; PCLS, precision-cut lung slices; PRO-C1 proteinase mediated release of the internal epitope in the N-terminal pro-peptide of type I collagen; PRO-C3, ADAMTS-2 mediated release of the N-terminal pro-peptide of type III collagen; PRO-C6, C-terminal of released C5 domain of type VI collagen α3 chain (endotrophin); SD, standard deviation; SEM, standard error of the mean
