Fig. 1
From: Endotracheal tube microbiome in hospitalized patients defined largely by hospital environment
Sampling strategy. ETT samples were collected and used for both analysis of microbial communities by sequencing of the 16S rRNA gene and for culturing in laboratory media. A section of the endotracheal tube was cut and immediately placed in 0.85% NaCl. Associated bacteria were dislodged by three cycles of vortex followed by sonication. DNA was extracted and the V3-V4 hypervariable region of the 16S rRNA gene was amplified and sequenced on the Illumina MiSeq platform