Fig. 5

Modulation of enhanced migratory capacity of HPS lung fibroblasts (HPSLF) by losartan in vivo. HPSLF and normal lung fibroblasts (NLF) were treated with losartan or vehicle (PBS) and labeled with a CM-Dil live-cell marker (red). 75 cells were injected into the yolk sac of 48 h post-fertilization Tg(fli1:GFP, green) zebrafish embryos expressing GFP in their blood vessels. HPSLFs exhibit stronger migratory capacity than NLFs at 24 h (not shown) and 48 h after injection. Arrows indicate the migration of labeled fibroblasts from the yolk sac into outlined regions of zebrafish embryos. Treatment with losartan reduces HPSLF migratory capacity compared to baseline