Fig. 1

Enhanced HPSLF migration in vitro is HPS1-dependent. A, F Representative microscopy images of scratch assays at 0, 12, and 24 h after gaps were generated. Scratch assays were performed with A NLF (n = 3 technical replicates) and HPSLF (n = 3 technical replicates); F NLF transfected with control or HPS1 siRNA; B, G Quantitative analysis of the migration potential of B NLF and HPSLF; G NLF transfected with control or HPS1 siRNA. The rate of fibroblast migration was determined by calculating the percentage of open wound area at the indicated time points to that of the corresponding initial scratch. C Ratio of CFSE MFI from proliferating cells over undivided cells D Western blot analysis of HPS1 protein expression in NLF transfected with either control or HPS1 siRNA. β-Actin was used as a loading control. E Ratio of HPS1 to β-Actin density expressed as fold change relative to NLF transfected with control siRNA. Data are expressed as mean ± SEM of three independent experiments. B, G Data analyzed using two-way mixed ANOVA *P < 0.05. C, E Data was analyzed using Student’s t-test *P < 0.05, **P < 0.01