Fig. 6

Direct anti-inflammatory effect of BGF treatment on lung endothelial cells via NFκB inhibition. Mouse lung endothelial cells (MLECs) were isolated via magnetic cell sorting, cultured until they reached ~ 90% confluence and treated with TNFα (10 ng/mL)/vehicle and with BGF/vehicle for 24 h. Then, the cells were harvested and further analyzed. A Representative immunoblotting of phospho-NFκB p65, total NFκB p65 and β-actin and the relative quantification (n = 3 biologically independent replicates) in MLECs from all the experimental groups. Blotting images were cropped between 75 and 50 kDa for phospho-NFκB p65 and total NFκB p65, and between 50 and 37 kDa for β-actin (B) Relative mRNA expression levels of TNFα, CXCL-8 and MMP2 in MLECs from all the experimental groups. The data are presented as the mean ± S.E.M. (standard error of mean). One-way ANOVA with Tukey’s post-hoc test was used for all figures