Fig. 1
From: Dehydroepiandrosterone in fibrotic interstitial lung disease: a translational study
Antifibrotic effect of DHEA in vitro and ex vivo. A Protein expression of EDA-FN and α-SMA in normal human lung fibroblasts treated with DHEA 150 µM (D150) and/or TGF-β1 (5 ng/ml) (T + D150) for 48 h. Bars represent mean ± SD, (*) p < 0.05, (**) p < 0.01. B Immunofluorescence images of DAPI (blue), Fibronectin (green), collagen I (red), and merged in human PCLS treated with control medium (CM), fibrotic cocktail (FC) and FC plus DHEA (FC + D150). Scale bar = 500 µm. α-SMA: alpha smooth muscle actin; CM: control medium; DAPI: diamidino-2-phenylindole; D150, dehydroepiandrosterone 150 µM; EDA-FN: extra domain A fibronectin; FC: fibrotic cocktail; RM: resting medium; SD: standard deviation; TGF-β1: transforming growth factor β1; T + D150: TGF- β1 + DHEA 150 µM