Fig. 7
From: Autophagy regulates the effects of ADSC-derived small extracellular vesicles on acute lung injury
The effect of ADSC-sEVs with or without autophagy inhibition on stress fiber formation. A Representative fluorescence images showing stress fibers labeled with F-actin staining. Nuclei were stained with DAPI. LPS significantly increased the formation of actin stress fibers, and this effect was significantly inhibited by sEVs. However, autophagy inhibition reduced the effect of sEVs on stress fiber formation. White arrows indicated the stress fibers labeled with F-actin. B Statistical analysis of the percentage of cells containing stress fibers in each experimental group. LPS treatment markedly increased the proportion of cells containing stress fibers, which was effectively decreased by ADSC-sEV treatment. However, autophagy inhibition weakened the effect of ADSC-sEVs on the formation of stress fibers (the percentage of cells containing stress fibers (%), control, 18.53 ± 1.31 vs. LPS, 61.97 ± 2.95 vs. LPS + ADSC-sEV, 35.13 ± 3.31 vs. LPS + ADSCsiATG5-sEV, 44.37 ± 3.09). The results are expressed as the mean ± SD of three independent experiments