Fig. 2

Characteristics of ADSC-sEVs. A Representative western blot analysis of small extracellular vesicles (sEVs) showing the presence of TSG 101 and CD9 but the absence of CD68 in ADSC-sEVs. B Nanoparticle tracking analysis of sEVs shows a single peak at 100 nm, and IL-1β preconditioning induced more sEVs production. C Electron microscopy showing the cup-shaped morphology of sEVs in both control and IL-1β-preconditioned ADSCs. D Representative western blot analysis of the expressions of TSG 101 and CD9 in ADSC-sEVs in presence or absence of IL-1β preconditioning. E Quantitative analysis of total amount protein in ADSC-sEVs in presence or absence of IL-1β preconditioning. IL-1β preconditioning promoted the production of these extracellular vesicles in ADSCs (quantification of the proteins in ADSC-sEVs (μg), control, 0.71 ± 0.03 vs. IL-1β, 0.95 ± 0.05). The results are expressed as the mean ± SD of three independent experiments