Fig. 6

AT7519, a CDK inhibitor, reverses neutrophil apoptosis in vitro. A, B Flow cytometry. GM-CSF and LPS delayed the neutrophil apoptosis and significantly increased cell viability. After AT7519 was added, the inhibitory effect of GM-CSF and LPS on neutrophil apoptosis was reversed (p < 0.0001, p = 0.002, respectively) and the ability to promote neutrophil survival was also weakened (p < 0.0001, p = 0.03, respectively). n = 3. C–F Western blot. When adding LPS and GM-CSF into medium, the level of p-GSK-3β (Ser9) increased, but in the presence of AT7519, the level of p-GSK-3β (Ser9) (D) was significantly reduced compared with the absence of AT7519, n = 4; changes in Mcl-1 (E) expression levels were consistent with that of p-GSK-3β (Ser9), n = 3; Cleaved-caspase 3 (F), a marker of apoptosis, increased when the medium contained AT7519, n = 3. The immunoblot data currently presented as separate groups were run on the same gel (with non-essential lanes removed). p values < 0.05 indicated statistical significance