Table 2 Overview of the two staining panels each dedicated to a specific cell type which is indicated by individual colours
Excitation (nm) | Blue: 488 | Red: 633 | Violent: 405 | |||||
Emission (nm) | 585 | 780 | 660 | 525 | 712 | 780 | 450 | 525 |
Fluorochrome | PE | PE-Cy7 | APC | AF488 | A700 | A750 | PB | KRO |
Panel/antibodies | CD25 | CD196 | CD127 | CD183 | CD8 | CD3 | CD4 | CD45 |
Volume of antibodies | 20 μL | 5 μL | 5 μL | 5 μL | 5 μL | 5 μL | 5 μL | 10 μL |