Fig. 1

CSE treatment differentially modulates the mRNA expression of PTGS2, PTGIS, and TBXAS1 in human PASMCs and PAECs. Confluent human PASMCs (A, C, and E) and PAECs (B, D, and F) were treated with different concentrations of CSE for 72 h and 24 h, respectively. Total RNA was isolated, and mRNA levels of PTGS2 (A, B), PTGIS (C, D), TBXAS1 (E, F), and the internal control β2M were determined by real-time RT- PCR. Results are calculated as the ratio of target gene mRNA and β2M mRNA and are expressed as fold change over untreated (0% CSE) cells. Each data point represents mean ± SEM from three independent experiments using PASMCs (A, C, and E) from three different donors and PAECs (B, D, and F) from one donor. *P < 0.05, **P < 0.01, ****P < 0.0001 compared with corresponding untreated cells