Fig. 6

Expression of the ten hub genes and screening in an independent dataset (GSE56153). A Scatter plot from multiple t tests. mRNA expression levels of ISG15, OAS1, IFIT3, OAS3, IFIT1, OASL, IFI44L, RSAD2, XAF1 and IFI44 in the peripheral blood from PTB patients were compared with those in HC groups. Data were analyzed with multiple t tests and the scatter plot is created via GraphPad Prism 8.0.2. Each dot represents one gene and red dots denote genes with significant changes in expression (q < 0.01). The X axis is the difference between means for each gene from PTB and HC groups. The Y value plots the minus logarithm of the q-value. A dotted grid line is shown at Y = −log10(0.01). B Correlation of ISG15, OAS1, IFIT3, OAS3, IFIT1, OASL, IFI44L, RSAD2, XAF1 and IFI44 expression levels and PTB variable. Correlations were analyzed using point-biserial correlation tests and correlation coefficients of these genes were plotted. Red circles denote genes with correlation coefficient > 0.5 and P < 0.01. C Forest plot of the association between the ten hub genes and PTB in GSE56153 dataset. These ten hub genes from PTB patients and HCs were analyzed using multivariate poisson regression analysis with robust variance estimate. Metaanalysis of those was conducted, and adjusted RR, 95% CI of each gene and corresponding P value were calculated and plotted in the forest plot. D mRNA expression values of the three genes (OAS1, IFIT1 and IFIT3) in PTB and HC from an independent sample set by qRT-PCR. The mRNA values of the evaluated genes were normalized to the housekeeping gene GAPDH. The numbers of participants in validation test were the following: PTB, n = 20; HC, n = 20. **P < 0.01, ***P < 0.001