Fig. 4
From: The role of retinoid-related orphan receptor-α in cigarette smoke-induced autophagic response
Effect of DRAM on RORα-dependent autophagy. A Beas-2B cells were exposed to 20% CSE for the indicated time. B Cells were exposed to the indicated concentrations of CSE for 4 h. Under these conditions, the total cell extracts were subjected to western blot using anti-DRAM and anti-α-actinin antibodies. C Primary lung fibroblasts were isolated from RORαWT and RORαsg/sg mice and treated with 20% CSE for the indicated time. D CSE-treated primary fibroblasts were exposed to 100 µM chloroquine for 4 h. E Beas-2B cells were transfected with control or DRAM siRNAs for 48 h. The cell lysates were prepared after treatment with 20% CSE for 4 h and subjected to western blot. F Beas-2B cells were transfected with the control or RORα siRNAs for 48 h and exposed to the indicated concentration of CSE. Cells were analyzed for their viability using an MTT assay. The data are expressed as the mean ± S.E. of 2 independent experiments with triplicate determinations