Fig. 3

CSE-induced autophagy is impaired in the fibroblasts from RORα^sg/sg mice. A Primary lung fibroblasts isolated from RORα^WT treated with 10% CSE for 4 h. B Primary lung fibroblasts were isolated from RORα^WT and RORα^sg/sg mice and treated with 10% CSE for the indicated time. C Autophagic flux in response to 10% CSE treatment was assessed based on the LC3 turnover using chloroquine in the fibroblasts from RORα^WT and RORα^sg/sg mice. D Transmission electron microscopy of the fibroblasts from RORα^WT and RORα^sg/sg mice treated with 10% CSE. Arrowheads indicate autophagosomes. E The number of autophagosomes per 10 µm² is shown