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Fig. 4 | Respiratory Research

Fig. 4

From: Transcriptional inhibition of miR-486-3p by BCL6 upregulates Snail and induces epithelial–mesenchymal transition during radiation-induced pulmonary fibrosis

Fig. 4

The siSnail inhibits IR-induced EMT and knockdown miR-486-3p-induced EMT. A Transfection of siSnail after irradiation of both cells using 6 Gy and detection of EMT-related protein expression. The bar graph shows the gray value analysis. B Transfection of miR-486-3p inhibitor and siSnail in both cells to examine changes of EMT-related proteins and the bargraph shows the gray value analysis. C In A549 cells, immunofluorescence experiments were used to analyze E-cadherin and N-cadherin changes in knockdown Snail after IR and IR. The histogram is a quantitative analysis of the relative fluorescence intensity and area. The scale bar represents 50 µm. D In A549, transfection with miR-486-3p and then knockdown of Snail was examined for changes in E-cadherin and N-cadherin. The scale bar represents 50 µm. On the right is the quantitative analysis of fluorescence intensity and area. *p < 0.05 compared with NC, #p < 0.05 compared with IR + NC or inhibitor + NC

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Respiratory Research

ISSN: 1465-993X

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