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Fig. 2 | Respiratory Research

Fig. 2

From: Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin

Fig. 2

Aerobic glycolysis was involved in HDM/lTSLP-induced TH2 Inflammatory cytokine release of airway epithelium. Airway epithelium 16-HBE was treatment with different concentration of HDM (A) and sTSLP (B) for 24 h and the indicators of aerobic glycolysis: glucose uptake (Glu), lactate dehydrogenase activity (LDHA) and lactate production (LD) were detected. C The expression levels of lTSLP and sTSLP protein were detected by western blot in HDM-induced 16-HBE. D The Glu, LDHA and LD were measured in 16-HBE with lTSLP treatment. E, F ELISA was used to measure inflammatory cytokine IL-25/33 in cell culture supernatant. G Western blots analysis showing lTSLP levels after lTSLP siRNA transfection in 16-HBE. H lTSLP levels in cell culture supernatant were measured by ELISA. I A series of metabolic parameters was measured for Glu, LDHA and LD in 16-HBE with lTSLP knockdown. *P < 0.05, n = 3 independent experiments

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Respiratory Research

ISSN: 1465-993X

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