Fig. 3

NIF inhibits pulmonary fibrosis induced by BLM. a Representative images showing H&E and Masson’s trichrome staining. Scale bars, 100 μm. b Quantification of fibrosis on lung sections based on the results of H&E staining; n = 5. c Quantification of collagen content volume fraction of lung sections based on Masson staining results; n = 5. d Representative images showing α-SMA, Collagen I and p-Stat3 staining of lung sections in mice. Scale bars, 50 µm. e Representative immunoblots of Collagen I, α-SMA, E-Cadherin, p-Stat3, Stat3 and β-actin in lung homogenates from mice as indicated. NIF (50 mg/kg). Statistical significance was evaluated using one-way ANOVA followed by Tukey’s test; All data are shown as the mean ± SD, ***P < 0.001 vs. Sham; ^###P < 0.001 vs. Vehicle